Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    The inhibitory influence of adipose tissue-derived mesenchymal stem cell environment and Wnt antagonism on breast tumour cell lines

    259605.pdf (712.0Kb)
    Access Status
    Open access
    Authors
    Visweswaran, M.
    Arfuso, Frank
    Dilley, R.
    Newsholme, P.
    Dharmarajan, Arunasalam
    Date
    2017
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Visweswaran, M. and Arfuso, F. and Dilley, R. and Newsholme, P. and Dharmarajan, A. 2017. The inhibitory influence of adipose tissue-derived mesenchymal stem cell environment and Wnt antagonism on breast tumour cell lines. International Journal of Biochemistry and Cell Biology. 95: pp. 63-72.
    Source Title
    International Journal of Biochemistry and Cell Biology
    DOI
    10.1016/j.biocel.2017.12.013
    ISSN
    1878-5875
    School
    School of Pharmacy and Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/60555
    Collection
    • Curtin Research Publications
    Abstract

    Tumours exhibit a heterogeneous mix of cell types that reciprocally regulate their growth in the tumour stroma, considerably affecting the progression of the disease. Both adipose-derived mesenchymal stem cells and Wnt signalling pathway are vital in driving breast tumour growth. Hence, we examined the effect of secreted factors released by adipose-derived mesenchymal stem cells, and further explored the anti-tumour property of the Wnt antagonist secreted frizzled-related protein 4 (sFRP4) on MCF-7 and MDA-MB-231 breast tumour cells. We observed that conditioned medium and extracellular matrix derived from adipose-derived mesenchymal stem cells inhibited tumour viability. The inhibitory effect of the conditioned medium was retained within its low molecular weight and non-protein component. The conditioned medium also induced apoptosis accompanied by a decrease in the mitochondrial membrane potential in tumour cells, Furthermore, it downregulated the protein expression of active ß-catenin and Cyclin D1, which are major target proteins of the Wnt signalling pathway, and reduced the expression of anti-apoptotic protein Bcl-xL. The combination of conditioned medium and sFRP4 further potentiated the effects, depending on the tumour cell line and experimental assay. We conclude that factors derived from conditioned medium of adipose-derived mesenchymal stem cells and sFRP4 significantly decreased the tumour cell viability and migration rates (MCF-7), accompanied with an enhanced apoptotic activity through inhibition of canonical Wnt signalling. Besides giving an insight to possible paracrine interactions and influence of signalling pathways, reflective of a breast tumour microenvironment, this study emphasises the utilization of cell free-secreted factors and Wnt antagonists to improve conventional anti-cancer strategies.

    Related items

    Showing items related by title, author, creator and subject.

    • The Influence of Breast Tumour-Derived Factors and Wnt Antagonism on the Transformation of Adipose-Derived Mesenchymal Stem Cells into Tumour-Associated Fibroblasts
      Visweswaran, M.; Keane, Kevin; Arfuso, Frank; Dilley, R.; Newsholme, Philip; Dharmarajan, Arunasalam (2018)
      Within the tumour stroma, a heterogeneous population of cell types reciprocally regulates cell proliferation, which considerably affects the progression of the disease. In this study, using tumour conditioned medium (TCM) ...
    • Implications of the WNT Signalling Pathway for Adipose-derived Mesenchymal Stem Cells in a Breast Tumour Environment
      Visweswaran, Malini (2017)
      This study describes the various roles adipose-derived mesenchymal stem cells (ADSCs) play in a breast tumour environment. The ADSC-derived secreted factors inhibit breast tumour cell growth aspects, whereas the tumour-derived ...
    • Regulation of cancer stem cell metabolism by secreted frizzled-related protein 4 (SFRP4)
      Deshmukh, Abhijeet; Arfuso, Frank; Newsholme, Philip; Dharmarajan, Arunasalam (2018)
      © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Tumours contain a small number of treatment-resistant cancer stem cells (CSCs), and it is through these that tumour regrowth originates at secondary sites, thus ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.