Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    External quality assessment of HLA-B*5701 reporting: An international multicentre survey

    Access Status
    Fulltext not available
    Authors
    Hammond, Emma
    Almeida, Coral-Ann
    Mamotte, Cyril
    Nolan, David
    Phillips, Elizabeth
    Schollaardt, Tineke Asma
    Gill, M. John
    Angel, Jonathan B.
    Neurath, Doris
    Li, Jianping
    Giulivi, Tony
    McIntyre, Cathy
    Koultchitski, Galina
    Wong, Betty
    Reis, Marciano
    Rachlis, Anita
    Cole, David E.
    Chew, Choo Beng
    Neifer, Stefan
    Lalonde, Richard
    Rogers, Michel
    Jeanneau, Annie
    Mallal, Simon
    Date
    2007
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Hammond, Emma and Almeida, Coral-Ann and Mamotte, Cyril and Nolan, David and Phillips, Elizabeth and Schollaardt, Tineke Asma and Gill, M. John et al. 2007. External quality assessment of HLA-B*5701 reporting: An international multicentre survey. Antiviral Therapy. 12 (7): 1027-1032.
    Source Title
    Antiviral Therapy
    Additional URLs
    http://www.intmedpress.com/serveFile.cfm?sUID=952ab0e5-e84f-4b9a-82ff-999e916f27c5
    ISSN
    1359 6535
    Faculty
    Division of Health Sciences
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/40639
    Collection
    • Curtin Research Publications
    Abstract

    Objectives: HLA-B*5701 strongly predicts abacavir hypersensitivity (HSR), but implementation of effective routine screening into clinical practice requires testing be practical and accurate. We tested the proficiency of HLA-B*5701 typing among laboratories using sequence specific primer PCR. Design and methods: DNA panels (1 and 2) were distributed to seven laboratories (A to G) for blinded typing of the HLA-B*5701 allele. Panel 1 (n=10 samples; n=7 laboratories) included 3 positives and other closely related B17 subtypes (B*5702, B*5703, B*5704 and B*5801). Panel 2 (n=96 samples; n=4 laboratories) included 36 positives among a broad spectrum of other B alleles. Two laboratories (A and B) also submitted 96 routine samples, typed by the same methodology, to the reference centre for additional analysis by sequence-based typing. Results: All laboratories correctly typed panel 1 for HLA-B*5701 carriage. Laboratories A, B and C identified HLA-B*5701 alleles in panel 2 with 100% sensitivity and 100% specificity. Laboratory D reported one false negative, reportedly due to a sampling error. The results obtained for routine samples typed by laboratories A and B and those generated by the reference laboratory using sequencing were fully concordant. Conclusions: Detection of HLA-B*5701 alleles among laboratories was 100% specific and 99.4% sensitive, indicating that participating HIV testing laboratories were currently offering effective primary screening to identify individuals at high risk of abacavir HSR. Accurate reporting of HLA-B*5701 status is critical for the safe administration of this drug and participation in quality assurance programmes by all sites who report HLA-B*5701 status should be promoted.

    Related items

    Showing items related by title, author, creator and subject.

    • HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay
      Hammond, E.; Cyril; Nolan, D.; Mallal, S. (2007)
      Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of ...
    • Characterization of tumor necrosis factor-a block haplotypes associated with susceptibility to chronic venous leg ulcers in Caucasian patients
      Tan, J.; Price, Patricia; Gut, I.; Stacey, M.; Warrington, N.; Wallace, H. (2010)
      Polymorphisms in the central major histocompatibility complex (MHC) are associated with several immunopathologic and inflammatory diseases, including chronic venous leg ulcers (CVLU). Because of strong linkage disequilibrium, ...
    • The investigation of the Staphylococcal Cassette Chromosome elements and Ciprofloxacin resistance in community Methicillin-Resistant Staphylococcus aureus Strains isolated in Western Australia
      Wilson, Lynne (2012)
      In Western Australia, community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) continues to be a public health concern. Antibiotic resistance places additional burdens on the community and health-care ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.