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    HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay

    Access Status
    Fulltext not available
    Authors
    Hammond, E.
    Cyril
    Nolan, D.
    Mallal, S.
    Date
    2007
    Type
    Journal Article
    
    Metadata
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    Citation
    Hammond, E. and Mamotte, C. and Nolan, D. and Mallal, S.. 2007. HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay. Tissue Antigens 70 (1): 58-61.
    Source Title
    Tissue Antigens
    DOI
    10.1111/j.1399-0039.2007.00840.x
    Faculty
    Division of Health Sciences
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/5945
    Collection
    • Curtin Research Publications
    Abstract

    Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods.

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