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    In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination

    Access Status
    Open access via publisher
    Authors
    Bustam, B.
    Dixon, Kingsley
    Bunn, E.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Bustam, B. and Dixon, K. and Bunn, E. 2014. In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination. Botanical Journal of the Linnean Society. 176 (4): pp. 556-566.
    Source Title
    Botanical Journal of the Linnean Society
    DOI
    10.1111/boj.12216
    ISSN
    0024-4074
    URI
    http://hdl.handle.net/20.500.11937/40753
    Collection
    • Curtin Research Publications
    Abstract

    Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of the plant growth regulators 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) or coconut water (CW) and compared their performance with germination on a standard symbiotic germination medium, oatmeal agar (OMA). Percentage germination of seeds every 2 weeks for a total of 8 weeks (five replicates per treatment), time to germination, and growth and development phases in seedlings were recorded. ½MS with 5% (v/v) fresh CW delivered 93% germination, with seedling vigour and development indistinguishable from OMA (95% germination). The same protocol was applied to a further ten species (including the endangered Caladenia huegelii), demonstrating high asymbiotic germination performance (60–93%) across a wide phylogenetic range of terrestrial orchid species.

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