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dc.contributor.authorBustam, B.
dc.contributor.authorDixon, Kingsley
dc.contributor.authorBunn, E.
dc.date.accessioned2017-01-30T14:45:29Z
dc.date.available2017-01-30T14:45:29Z
dc.date.created2015-06-10T20:00:53Z
dc.date.issued2014
dc.identifier.citationBustam, B. and Dixon, K. and Bunn, E. 2014. In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination. Botanical Journal of the Linnean Society. 176 (4): pp. 556-566.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/40753
dc.identifier.doi10.1111/boj.12216
dc.description.abstract

Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of the plant growth regulators 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) or coconut water (CW) and compared their performance with germination on a standard symbiotic germination medium, oatmeal agar (OMA). Percentage germination of seeds every 2 weeks for a total of 8 weeks (five replicates per treatment), time to germination, and growth and development phases in seedlings were recorded. ½MS with 5% (v/v) fresh CW delivered 93% germination, with seedling vigour and development indistinguishable from OMA (95% germination). The same protocol was applied to a further ten species (including the endangered Caladenia huegelii), demonstrating high asymbiotic germination performance (60–93%) across a wide phylogenetic range of terrestrial orchid species.

dc.publisherWiley-Blackwell
dc.titleIn vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination
dc.typeJournal Article
dcterms.source.volume176
dcterms.source.number4
dcterms.source.startPage556
dcterms.source.endPage566
dcterms.source.issn0024-4074
dcterms.source.titleBotanical Journal of the Linnean Society
curtin.accessStatusOpen access via publisher


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