Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal
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2012Type
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Endo, T. and Henares, B. and Hisamoto, H. 2012. Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal, in Fujii, T. (ed), Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Oct 28-Nov 1 2012, pp. 950-952. Okinawa, Japan: Chemical and Biological Microsystems Society.
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Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
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Centre for Crop Disease Management
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Abstract
The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions.
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