Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal
Access Status
Fulltext not available
Authors
Endo, T.
Henares, Bernadette
Hisamoto, H.
Date
2012Type
Conference Paper
Metadata
Show full item recordCitation
Endo, T. and Henares, B. and Hisamoto, H. 2012. Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal, in Fujii, T. (ed), Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Oct 28-Nov 1 2012, pp. 950-952. Okinawa, Japan: Chemical and Biological Microsystems Society.
Source Title
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
ISBN
School
Centre for Crop Disease Management
Collection
Abstract
The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions.
Related items
Showing items related by title, author, creator and subject.
-
Mor, I.; Carlessi, Rodrigo; Ast, T.; Feinstein, E.; Kimchi, A. (2012)Death-associated protein kinase (DAPk), a multi-domain serine/threonine kinase, regulates numerous cell death mechanisms and harbors tumor suppressor functions. In this study, we report that DAPk directly binds and ...
-
Fyffe, C.; Falasca, Marco (2013)It should be noted that 3-phosphoinositide-dependent protein kinase-1 (PDK1) is a protein encoded by the PDPK1 gene, which plays a key role in the signaling pathways activated by several growth factors and hormones. PDK1 ...
-
Donoghue, Alan M. (2000)Objectives - To examine the incidence, clinical state, personal risk factors, haematology and biochemistry of heat exhaustion cases occurring at a deep underground metalliferous mine. To describe the underground and surface ...