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    Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal

    Access Status
    Fulltext not available
    Authors
    Endo, T.
    Henares, Bernadette
    Hisamoto, H.
    Date
    2012
    Type
    Conference Paper
    
    Metadata
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    Citation
    Endo, T. and Henares, B. and Hisamoto, H. 2012. Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal, in Fujii, T. (ed), Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Oct 28-Nov 1 2012, pp. 950-952. Okinawa, Japan: Chemical and Biological Microsystems Society.
    Source Title
    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    ISBN
    9780979806452
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/41465
    Collection
    • Curtin Research Publications
    Abstract

    The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions.

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