Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal
| dc.contributor.author | Endo, T. | |
| dc.contributor.author | Henares, Bernadette | |
| dc.contributor.author | Hisamoto, H. | |
| dc.date.accessioned | 2017-01-30T14:51:59Z | |
| dc.date.available | 2017-01-30T14:51:59Z | |
| dc.date.created | 2015-10-29T04:09:24Z | |
| dc.date.issued | 2012 | |
| dc.identifier.citation | Endo, T. and Henares, B. and Hisamoto, H. 2012. Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal, in Fujii, T. (ed), Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Oct 28-Nov 1 2012, pp. 950-952. Okinawa, Japan: Chemical and Biological Microsystems Society. | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/41465 | |
| dc.description.abstract |
The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions. | |
| dc.publisher | Chemical and Biological Microsystems Society | |
| dc.title | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal | |
| dc.type | Conference Paper | |
| dcterms.source.startPage | 950 | |
| dcterms.source.endPage | 952 | |
| dcterms.source.title | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | |
| dcterms.source.series | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | |
| dcterms.source.isbn | 9780979806452 | |
| curtin.department | Centre for Crop Disease Management | |
| curtin.accessStatus | Fulltext not available |
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