The impact of cryopreservation on human peripheral blood leucocyte bioenergetics

Keane, Kevin; Calton, E.; Cruzat, Vinicius; Soares, M.; Newsholme, Philip

doi:10.1042/cs20140725

Access Status

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Authors

Keane, Kevin

Calton, E.

Cruzat, Vinicius

Soares, M.

Newsholme, Philip

Date

2015

Type

Journal Article

Metadata

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Citation

Keane, K. and Calton, E. and Cruzat, V. and Soares, M. and Newsholme, P. 2015. The impact of cryopreservation on human peripheral blood leucocyte bioenergetics. Clinical Science. 128 (10): pp. 723-733.

Source Title

Clinical science (London, England : 1979)

DOI

10.1042/cs20140725

School

School of Biomedical Sciences

URI

http://hdl.handle.net/20.500.11937/42333

Collection

Curtin Research Publications

Abstract

This study has demonstrated for the first time that cryopreservation of primary immune cells modified their metabolism in a time-dependent fashion, indicated by attenuated aerobic respiration and enhanced glycolytic activity. Measurements were recorded using the Seahorse XFe96 extracellular flux analyser.