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Characterization of the sheep Complement Factor B gene (CFB)

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Open access
Authors
Qin, J
Munyard, Kylie
Lee, C
Wetherall, John
Groth, David
Date
2011
Type
Journal Article

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Qin, J and Munyard, Kylie and Lee, C and Wetherall, John and Groth, David. 2011. Characterization of the sheep Complement Factor B gene (CFB). Veterinary Immunology and Immunopathology. 140 (1-2): pp. 170-174.
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Veterinary Immunology and Immunopathology
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School of Biomedical Sciences
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NOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Immunology and Immunopathology, 140, 1-2, 2011. DOI: 10.1016/j.vetimm.2010.11.008

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Abstract

The Complement Factor B gene (CFB) of the alternative complement pathway has been identified in the sheep Major Histocompatibility Complex (MHC) and its genomic sequence determined. CFB is located approximately 600bp upstream of the complement C2 gene, contains 18 exons, and manifests the domain signature characteristic of CFB protein. Thirteen single nucleotide polymorphisms were identified in merino sheep and interbreed variation was identified by comparison with International Sheep Genomics Consortium data. Two predicted non synonymous substitutions were observed and in-silico analysis indicates that these are likely to have a destabilising effect on the protein structure. Sheep and cattle CFB were compared and shown to contain a common nine nucleotide deletion in exon 18 relative to human CFB. Predicted CFB amino acid sequences for these two species contain 761 aa relative to 764 aa in the human orthologue. Sequencing of the cosmid and BAC clones used in this study permitted the relative positions of three adjacent loci to be determined and showed that the previously described microsatellite locus (BfMs) is located within SKIV2L.