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dc.contributor.authorQin, J
dc.contributor.authorMunyard, Kylie
dc.contributor.authorLee, C
dc.contributor.authorWetherall, John
dc.contributor.authorGroth, David
dc.date.accessioned2017-01-30T14:59:30Z
dc.date.available2017-01-30T14:59:30Z
dc.date.created2011-09-19T20:01:37Z
dc.date.issued2011
dc.identifier.citationQin, J and Munyard, Kylie and Lee, C and Wetherall, John and Groth, David. 2011. Characterization of the sheep Complement Factor B gene (CFB). Veterinary Immunology and Immunopathology. 140 (1-2): pp. 170-174.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/42421
dc.identifier.doi10.1016/j.vetimm.2010.11.008
dc.description.abstract

The Complement Factor B gene (CFB) of the alternative complement pathway has been identified in the sheep Major Histocompatibility Complex (MHC) and its genomic sequence determined. CFB is located approximately 600bp upstream of the complement C2 gene, contains 18 exons, and manifests the domain signature characteristic of CFB protein. Thirteen single nucleotide polymorphisms were identified in merino sheep and interbreed variation was identified by comparison with International Sheep Genomics Consortium data. Two predicted non synonymous substitutions were observed and in-silico analysis indicates that these are likely to have a destabilising effect on the protein structure. Sheep and cattle CFB were compared and shown to contain a common nine nucleotide deletion in exon 18 relative to human CFB. Predicted CFB amino acid sequences for these two species contain 761 aa relative to 764 aa in the human orthologue. Sequencing of the cosmid and BAC clones used in this study permitted the relative positions of three adjacent loci to be determined and showed that the previously described microsatellite locus (BfMs) is located within SKIV2L.

dc.publisherElsevier BV
dc.subjectCattle
dc.subjectCFB
dc.subjectSheep
dc.subjectComplement
dc.subjectSNP
dc.subjectMHC
dc.titleCharacterization of the sheep Complement Factor B gene (CFB)
dc.typeJournal Article
dcterms.source.volume140
dcterms.source.number1-2
dcterms.source.startPage170
dcterms.source.endPage174
dcterms.source.issn01652427
dcterms.source.titleVeterinary Immunology and Immunopathology
curtin.note

NOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Immunology and Immunopathology, 140, 1-2, 2011. DOI: 10.1016/j.vetimm.2010.11.008

curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access


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