Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri

Shao, P.; Liu, J.; Chen, X.; Fang, Zhongxiang; Sun, P.

doi:10.1016/j.ijbiomac.2014.10.056

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Authors

Shao, P.

Liu, J.

Chen, X.

Fang, Zhongxiang

Sun, P.

Date

2015

Type

Journal Article

Metadata

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Citation

Shao, P. and Liu, J. and Chen, X. and Fang, Z. and Sun, P. 2015. Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri. International Journal of Biological Macromolecules. 73: pp. 124-130.

Source Title

International Journal of Biological Macromolecules

DOI

10.1016/j.ijbiomac.2014.10.056

ISSN

0141-8130

School

School of Public Health

URI

http://hdl.handle.net/20.500.11937/43312

Collection

Curtin Research Publications

Abstract

A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78 × 105 Da and composed of T-d-Glcp, 1,3-d-Glcp, 1,6-d-Glcp and 1,3,6-d-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1 → 6) side chains linked to a β-(1 → 3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent.