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A regulatory toolbox of MiniPromoters to drive selective expression in the brain

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Open access via publisher
Authors
Portales-Casamar, E.
Swanson, D.
Liu, L.
De Leeuw, C.
Banks, K.
Ho Sui, S.
Fulton, D.
Ali, J.
Amirabbasi, M.
Arenillas, D.
Babyak, N.
Black, S.
Bonaguro, R.
Brauer, E.
Candido, T.
Castellarin, M.
Chen, J.
Chen, Y.
Cheng, J.
Chopra, V.
Docking, T.
Dreolini, L.
D'Souza, C.
Flynn, E.
Glenn, R.
Hatakka, K.
Hearty, T.
Imanian, B.
Jiang, S.
Khorasan-zadeh, S.
Komljenovic, I.
Laprise, S.
Liao, N.
Lim, J.
Lithwick, S.
Liu, F.
Liu, J.
Lu, M.
McConechy, M.
McLeod, A.
Milisavljevic, M.
Mis, J.
O'Connor, K.
Palma, B.
Palmquist, D.
Schmouth, J.
Swanson, M.
Tam, B.
Ticoll, A.
Turner, J.
Varhol, Richard
Vermeulen, J.
Watkins, R.
Wilson, G.
Wong, B.
Wong, S.
Wong, T.
Yang, G.
Ypsilanti, A.
Jones, S.
Holt, R.
Goldowitz, D.
Wasserman, W.
Simpson, E.
Date
2010
Type
Journal Article

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Citation
Portales-Casamar, E. and Swanson, D. and Liu, L. and De Leeuw, C. and Banks, K. and Ho Sui, S. and Fulton, D. et al. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proceedings of the National Academy of Sciences of USA. 107 (38): pp. 16589-16594.
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Proceedings of the National Academy of Sciences of USA
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Department of Health Policy and Management
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Abstract

The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination "knockins" in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5' of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or inmouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type - specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies.