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    Identification of a set of genes showing regionally enriched expression in the mouse brain

    Access Status
    Open access via publisher
    Authors
    D'Souza, C.
    Chopra, V.
    Varhol, Richard
    Xie, Y.
    Bohacec, S.
    Zhao, Y.
    Lee, L.
    Bilenky, M.
    Portales-Casamar, E.
    He, A.
    Wasserman, W.
    Goldowitz, D.
    Marra, M.
    Holt, R.
    Simpson, E.
    Jones, S.
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    D'Souza, C. and Chopra, V. and Varhol, R. and Xie, Y. and Bohacec, S. and Zhao, Y. and Lee, L. et al. 2008. Identification of a set of genes showing regionally enriched expression in the mouse brain. BMC Neuroscience. 9.
    Source Title
    BMC Neuroscience
    DOI
    10.1186/1471-2202-9-66
    School
    Department of Health Policy and Management
    URI
    http://hdl.handle.net/20.500.11937/32990
    Collection
    • Curtin Research Publications
    Abstract

    Background: The Pleiades Promoter Project aims to improve gene therapy by designing human mini-promoters (< 4 kb) that drive gene expression in specific brain regions or cell-types of therapeutic interest. Our goal was to first identify genes displaying regionally enriched expression in the mouse brain so that promoters designed from orthologous human genes can then be tested to drive reporter expression in a similar pattern in the mouse brain. Results: We have utilized LongSAGE to identify regionally enriched transcripts in the adult mouse brain. As supplemental strategies, we also performed a meta-analysis of published literature and inspected the Allen Brain Atlas in situ hybridization data. From a set of approximately 30,000 mouse genes, 237 were identified as showing specific or enriched expression in 30 target regions of the mouse brain. GO term over-representation among these genes revealed co-involvement in various aspects of central nervous system development and physiology. Conclusion: Using a multi-faceted expression validation approach, we have identified mouse genes whose human orthologs are good candidates for design of mini-promoters. These mouse genes represent molecular markers in several discrete brain regions/cell-types, which could potentially provide a mechanistic explanation of unique functions performed by each region. This set of markers may also serve as a resource for further studies of gene regulatory elements influencing brain expression. © 2008 D'Souza et al; licensee BioMed Central Ltd.

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