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    Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors

    Access Status
    Fulltext not available
    Authors
    Guan, B.
    Magenau, A.
    Kilian, K.
    Ciampi, Simone
    Gaus, K.
    Reece, P.
    Gooding, J.
    Date
    2011
    Type
    Journal Article
    
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    Citation
    Guan, B. and Magenau, A. and Kilian, K. and Ciampi, S. and Gaus, K. and Reece, P. and Gooding, J. 2011. Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors. Faraday Discussions. 149: pp. 301-317.
    Source Title
    Faraday Discussions
    DOI
    10.1039/c005340f
    ISSN
    1359-6640
    School
    Nanochemistry Research Institute
    URI
    http://hdl.handle.net/20.500.11937/4499
    Collection
    • Curtin Research Publications
    Abstract

    In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and recognition molecules (antibodies) such that these devices could potentially be used for single-cell identification or sensing. This is achieved by modifying PSi Rugate filters via hydrosilylation with surface chemistry that serves firstly, to protect the silicon surfaces from oxidation; secondly, renders the surfaces resistant to nonspecific adsorption of proteins and cells and thirdly, allows further functionality to be added such as the coupling of antibodies. The surface chemistry remained unchanged after sonication of the PSi to form PSi microparticles. The ability to monitor the spectroscopic properties of microparticles, and shifts in the optical signature due to changes in the refractive index of the material within the pore space, is demonstrated. The particles are shown to remain stable in physiological buffers and human blood for longer than one week. Finally, the modification of the PSi particles with functional antibodies is achieved. © 2011 The Royal Society of Chemistry.

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