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dc.contributor.authorBhuvanalakshmi, G.
dc.contributor.authorArfuso, Frank
dc.contributor.authorDharmarajan, Arunasalam
dc.contributor.authorWarrier, Sudha
dc.date.accessioned2017-01-30T15:20:02Z
dc.date.available2017-01-30T15:20:02Z
dc.date.created2015-03-18T20:00:25Z
dc.date.issued2014
dc.identifier.citationBhuvanalakshmi, G. and Arfuso, F. and Dharmarajan, A. and Warrier, S. 2014. Multifunctional Properties of Chicken Embryonic Prenatal Mesenchymal Stem Cells- Pluripotency, Plasticity, and Tumor Suppression. Stem Cell Reviews and Reports. 10 (6): pp. 856-870.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/45328
dc.identifier.doi10.1007/s12015-014-9530-3
dc.description.abstract

The chick embryo represents an accessible and economical in vivo model, which has long been used in developmental biology, gene expression analysis, and loss/gain of function experiments. In the present study, we assessed and characterized bone marrow derived mesenchymal stem cells from prenatal day 13 chicken embryos (chBMMSCs) and determined some novel properties. After assessing the mesenchymal stem cell (MSC) properties of these cells by the presence of their signature markers (CD 44, CD 73, CD 90, CD 105, and vimentin), we ascertained a very broad spectrum of multipotentiality as these MSCs not only differentiated into the classic tri-lineages of MSCs but also into ectodermal, endodermal, and mesodermal lineages such as neuron, hepatocyte, islet cell, and cardiac. In addition to wide plasticity, we detected the presence of several pluripotent markers such as Oct4, Sox2, and Nanog. This is the first study characterizing prenatal chBMMSCs and their ability to not only differentiate into mesenchymal lineages but also into all the germ cell layer lineages. Furthermore, our studies indicate that prenatal chBMMSCs derived from the chick provide an excellent model for multi-lineage development studies because of their broad plasticity and faithful reproduction of MSC traits as seen in the human. Here, we also present evidence for the first time that media derived from prenatal chBMMSC cultures have an anti-tumorigenic, anti-migratory, and pro-apoptotic effect on human tumors cells acting through the Wnt-ß-catenin pathway. These data confirm that chBMMSCs are enriched with factors in their secretome that are able to destroy tumor cells. This suggests a commonality of properties of MSCs across species between human and chicken.

dc.publisherSpringer US
dc.subjectMulti-lineage differentiation
dc.subjectTumor suppression
dc.subjectChicken
dc.subjectMesenchymal stemcells
dc.subjectPluripotency
dc.titleMultifunctional Properties of Chicken Embryonic Prenatal Mesenchymal Stem Cells- Pluripotency, Plasticity, and Tumor Suppression
dc.typeJournal Article
dcterms.source.volume10
dcterms.source.number6
dcterms.source.startPage856
dcterms.source.endPage870
dcterms.source.issn1550-8943
dcterms.source.titleStem Cell Reviews and Reports
curtin.note

The final publication is available at Springer via http://doi.org/10.1007/s12015-014-9530-3

curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access


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