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    Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae)

    242019_242019.pdf (511.0Kb)
    Access Status
    Open access
    Authors
    Nevill, Paul
    Wardell-Johnson, G.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Nevill, P. and Wardell-Johnson, G. 2016. Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae). Applications in Plant Sciences. 4 (11): 1600084.
    Source Title
    Appl Plant Sci
    DOI
    10.3732/apps.1600084
    ISSN
    2168-0450
    School
    Department of Environment and Agriculture
    Funding and Sponsorship
    http://purl.org/au-research/grants/arc/IC150100041
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by-nc-sa/4.0/

    URI
    http://hdl.handle.net/20.500.11937/45792
    Collection
    • Curtin Research Publications
    Abstract

    Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85. Conclusions: These markers will enable the quantification of genetic impact of proposed mining activities on the short-range endemic Acacia adinophylla.

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