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dc.contributor.authorNevill, Paul
dc.contributor.authorWardell-Johnson, G.
dc.date.accessioned2017-01-30T15:23:22Z
dc.date.available2017-01-30T15:23:22Z
dc.date.created2016-08-17T19:30:20Z
dc.date.issued2016
dc.identifier.citationNevill, P. and Wardell-Johnson, G. 2016. Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae). Applications in Plant Sciences. 4 (11): 1600084.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/45792
dc.identifier.doi10.3732/apps.1600084
dc.description.abstract

Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85. Conclusions: These markers will enable the quantification of genetic impact of proposed mining activities on the short-range endemic Acacia adinophylla.

dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/IC150100041
dc.titleMicrosatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae)
dc.typeJournal Article
dcterms.source.volumeTBA
dcterms.source.startPageTBS
dcterms.source.endPageTBS
dcterms.source.issn2168-0450
dcterms.source.titleAppl Plant Sci
curtin.note

This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by-nc-sa/4.0/

curtin.departmentDepartment of Environment and Agriculture
curtin.accessStatusOpen access


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