Ion Channel Mimetic Chronopotentiometric Polymeric Membrane Ion Sensor for Surface-Confined Protein Detection
MetadataShow full item record
The operation of ion channel sensors is mimicked with functionalized polymeric membrane electrodes, using a surface confined affinity reaction to impede the electrochemically imposed ion transfer kinetics of a marker ion. A membrane surface biotinylated by covalent attachment to the polymeric backbone is used here to bind to the protein avidin as a model system. The results indicate that the protein accumulates on the ion-selective membrane surface, partially blocking the current-induced ion transfer across the membrane/aqueous sample interface, and subsequently decreases the potential jump in the so-called super-Nernstian step that is characteristic of a surface depletion of the marker ion. The findings suggest that such a potential drop could be utilized to measure the concentration of protein in the sample. Because the sensitivity of protein sensing is dependent on the effective blocking of the active surface area, it can be improved with a hydrophilic nanopore membrane applied on top of the biotinylated ion-selective membrane surface. On the basis of cyclic voltammetry characterization, the nanoporous membrane electrodes can indeed be understood as a recessed nanoelectrode array. The results show that the measuring range for protein sensing on nanopore electrodes is shifted to lower concentrations by more than 1 order of magnitude, which is explained with the reduction of surface area by the nanopore membrane and the related more effective hemispherical diffusion pattern.
Open access to this article will be available 12 months after publication via the website of the American Chemical Society : http://pubs.acs.org
Showing items related by title, author, creator and subject.
Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid HybridizationEllis, J.; Herzog, G.; Glynn, B.; Arrigan, Damien (2011)We report on the electrochemical characterization of regularlyaligned cylindrical nanopore arrays supported in silicon nitride membranes and preliminary results for the detection of nucleic acid hybridization on the ...
Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line: A Preliminary StudyPoinern, G.; Le, X.; O'Dea, M.; Becker, Thomas; Fawcett, D. (2014)In this preliminary study we investigate for the first time the biomedical potential of using porous anodic aluminium oxide (AAO) membranes as a cell substrate for culturing the Cercopithecus aethiops (African green monkey) ...
Porous graphene nanosheets functionalized thin film nanocomposite membrane prepared by interfacial polymerization for CO<inf>2</inf>/N<inf>2</inf>separationLi, H.; Ding, X.; Zhang, Y.; Liu, Jian (2017)© 2017 Elsevier B.V. The inherent defects of porous graphene (PG) formed during reduction etching process could serve as nanopores, making PG emerge a potential application for the preparation of micrometre-sized separation ...