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dc.contributor.authorTan, S.
dc.contributor.authorAcquah, Caleb
dc.contributor.authorSidhu, Amandeep
dc.contributor.authorOngkudon, Clarence
dc.contributor.authorYon, L.S.
dc.contributor.authorDanquah, Michael
dc.date.accessioned2017-01-30T10:40:23Z
dc.date.available2017-01-30T10:40:23Z
dc.date.created2016-05-25T19:30:16Z
dc.date.issued2016
dc.identifier.citationTan, S. and Acquah, C. and Sidhu, A. and Ongkudon, C. and Yon, L. Danquah, M. 2016. SELEX modifications and bioanalytical techniques for Aptamer-target binding characterization. Critical Reviews in Analytical Chemistry. 46 (6): pp. 521-537.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/4619
dc.identifier.doi10.1080/10408347.2016.1157014
dc.description.abstract

The quest to improve the detection of biomolecules and cells in health and life sciences has led to the discovery and characterization of various affinity bioprobes. Libraries of synthetic oligonucleotides (ssDNA/ssRNA) with randomized sequences are employed during Systematic Evolution of Ligands by Exponential Enrichment (SELEX) to select highly specific affinity probes called aptamers. With much focus on the generation of aptamers for a variety of target molecules, conventional SELEX protocols have been modified to develop new and improved SELEX protocols yielding highly specific and stable aptamers. Various techniques have been used to analyze the binding interactions between aptamers and their cognate molecules with associated merits and limitations. This article comprehensively reviews research advancements in the generation of aptamers, analyses physicochemical conditions affecting their binding characteristics to cellular and biomolecular targets, and discusses various field applications of aptameric binding. Biophysical techniques employed in the characterization of the molecular and binding features of aptamers to their cognate targets are also discussed.

dc.titleSELEX modifications and bioanalytical techniques for aptamer-target binding characterization
dc.typeJournal Article
dcterms.source.volume46
dcterms.source.startPage521
dcterms.source.endPage537
dcterms.source.titleCrit Rev Anal Chem
curtin.note

This is an Author's Original Manuscript of an article published by Taylor & Francis in Critical Reviews in Analytical Chemistry on 15/03/2016 available online at <a href="http://www.tandfonline.com/10.1080/10408347.2016.1157014">http://www.tandfonline.com/10.1080/10408347.2016.1157014</a>

curtin.departmentCurtin Sarawak
curtin.accessStatusFulltext not available


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