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dc.contributor.authorBlack, Lucinda
dc.contributor.authorAnderson, D.
dc.contributor.authorClarke, M.
dc.contributor.authorPonsonby, A.-L.
dc.contributor.authorLucas, R.
dc.date.accessioned2017-01-30T15:29:27Z
dc.date.available2017-01-30T15:29:27Z
dc.date.created2016-04-28T19:30:18Z
dc.date.issued2015
dc.identifier.citationBlack, L. and Anderson, D. and Clarke, M. and Ponsonby, A.-L. and Lucas, R. 2015. Analytical bias in the measurement of serum 25-hydroxyvitamin D concentrations impairs assessment of vitamin D status in clinical and research settings. PLoS One. 10 (8): Article ID e0135478.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/46832
dc.identifier.doi10.1371/journal.pone.0135478
dc.description.abstract

Measured serum 25-hydroxyvitamin D concentrations vary depending on the type of assay used and the specific laboratory undertaking the analysis, impairing the accurate assessment of vitamin D status. We investigated differences in serum 25-hydroxyvitamin D concentrations measured at three laboratories (laboratories A and B using an assay based on liquid chromatography-tandem mass spectrometry and laboratory C using a DiaSorin Liaison assay), against a laboratory using an assay based on liquid chromatography-tandem mass spectrometry that is certified to the standard reference method developed by the National Institute of Standards and Technology and Ghent University (referred to as the ‘ certified laboratory ’ ). Separate aliquots from the same original serum sample for a subset of 50 participants from the Ausimmune Study were analysed at the four laboratories. Bland-Altman plots were used to visually check agreement between each laboratory against the certified laboratory. Compared with the certified laboratory, serum 25-hydroxyvitamin D concentrations were on average 12.4 nmol/L higher at laboratory A (95% limits of agreement: -17 .8,42.6); 12.8 nmol/L higher at laboratory B (95% limits of agreement: 0.8,24.8); and 10.6 nmol/L lower at laboratory C (95% limits of agreement: -48.4,27.1). The prevalence of vitamin D deficiency (defined here as 25-hydroxyvitamin D < 50 nmol/L) was 24%, 16%, 12% and 41% at the certified laboratory, and laboratories A, B, and C, respectively. Our results demonstrate considerable differences in the measurement of 25-hydroxyvitamin D concentrations compared with a certified laboratory, even between laboratories using assays based on liquid chromatography-tandem mass spectrometry, which is often considered the gold-standard assay. To ensure accurate and reliable measurement of serum 25-hydroxyvitamin D concentrations, all laboratories should use an accuracy-based quality assurance system and, ideally, comply with international standardisation efforts

dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleAnalytical bias in the measurement of serum 25-hydroxyvitamin D concentrations impairs assessment of vitamin D status in clinical and research settings
dc.typeJournal Article
dcterms.source.volume10
dcterms.source.number8
dcterms.source.startPage1
dcterms.source.endPage14
dcterms.source.issn1932-6203
dcterms.source.titlePLoS One
curtin.departmentSchool of Public Health
curtin.accessStatusOpen access


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