Evidence that the cAMP Pathway Controls Emergence of Both Primary and Appressorial Germ Tubes of Barley Powdery Mildew
MetadataShow full item record
A copy of this item may be available from Professor Richard Oliver
Development of conidia of barley powdery mildew involves the formation of a primary germ tube (PGT), an appressorial germ tube (AGT), and an appressorium. Previously, it was found that cyclic AMP (cAMP) was involved in these developmental processes. Comparison of development on the host surface with two types of cellulose membrane revealed that frequency of PGT emergence was surface independent. On one type of cellulose, where the frequencies of both AGT and appressorial differentiation were similar to that on the host surface, cAMP levels and protein kinase A (PKA) activities had a biphasic pattern with peaks at 15 min and 4 h after inoculation (prior to PGT and AGT emergence, respectively). The effect of manipulating cAMP levels was tested on another type of cellulose membrane, which stimulated a lower degree of AGT and appressorial formation than the host surface. Cholera toxin and forskolin, activators of adenylyl cyclase, significantly increased PGT emergence, but cAMP did not. Cholera toxin, forskolin, and cAMP increased the frequency of AGT and appressorial formation, but in a time-dependent manner.
Showing items related by title, author, creator and subject.
Evidence that the Appressorial Development in Barley Powdery Mildew is Controlled by MAP Kinase Activity in Conjunction with the cAMP PathwayKinane, J.; Oliver, Richard (2003)Development of the barley powdery mildew fungus involves the sequential formation of a primary germ tube, an appressorial germ tube, and an appressorium. Previously, we have shown that the cAMP pathway controls the emergence ...
Involvement of cAMP and Protein Kinase A in Conidial Differentiation by Erysiphe graminis f. sp. hordeiHall, A.; Bindslev, L.; Rouster, J.; Rasmussen, S.; Oliver, Richard; Gurr, S. (1999)Erysiphe graminis f. sp. hordei, the causal agent of barley powdery mildew, is an obligate biotroph. On arrival on the host, a primary germ tube (PGT) emerges from the conidium. An appressorial germ tube (AGT) then appears, ...
Transcript Profiling in the Barley Mildew Pathogen Blumeria graminis by Serial Analysis of Gene Expression (SAGE)Thomas, S.; Glaring, M.; Rasmussen, S.; Kinane, J.; Oliver, Richard (2002)The fungal pathogen Blumeria graminis f. sp. hordei develops on the barley leaf via distinct, morphologically well-defined stages. After landing on a host plant, the conidia rapidly germinate to form a primary germ tube. ...