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    Involvement of cAMP and Protein Kinase A in Conidial Differentiation by Erysiphe graminis f. sp. hordei

    Access Status
    Fulltext not available
    Authors
    Hall, A.
    Bindslev, L.
    Rouster, J.
    Rasmussen, S.
    Oliver, Richard
    Gurr, S.
    Date
    1999
    Type
    Journal Article
    
    Metadata
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    Citation
    HALL AA, BINDSLEV L, ROUSTER J, RASMUSSEN SW, OLIVER RP & GURR SJ (1999) Involvement of cAMP and Protein Kinase A in Conidial Differentiation by Erysiphe graminis f. sp. hordei. Molecular Plant Microbe Interactions 12 960-968
    DOI
    10.1094/MPMI.1999.12.11.960
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/5779
    Collection
    • Curtin Research Publications
    Abstract

    Erysiphe graminis f. sp. hordei, the causal agent of barley powdery mildew, is an obligate biotroph. On arrival on the host, a primary germ tube (PGT) emerges from the conidium. An appressorial germ tube (AGT) then appears, forms an appressorium, and effects host penetration. Such developmental precision may be due to multiple, plant-derived signals and to endogenous tactile and chemical signals. The transduction mechanism remains obscure. The isolation of an expressed sequence tag (EST) homologue of the catalytic subunit of cyclic AMP (cAMP)-dependent protein kinase A (PKA) enabled the corresponding gene to be characterized and the transcript to be identified in conidia and in PGT and AGT stage spores. cAMP-dependent PKA activity was detected in ungerminated conidia. These data suggest that PKA and cAMP are involved in conidial development. To substantiate this we exploited the responses of developing conidia to various surfaces, including exposure to the host leaf (fully inductive to AGT formation), cellulose membrane (semi-inductive), and glass (non-inductive). Assessment of fungal development, following application of exogenous cAMP or cAMP analogues, revealed that, at different concentrations and on different surfaces, cAMP either promoted or inhibited conidial differentiation. Various PKA inhibitors were tested for their effect on PKA activity and conidial development. A negative correlation was established between PKA inhibition in vitro and fungal development in vivo. Taken collectively, these data suggest that PKA and cAMP play a role in conidial differentiation in this obligate, plant-pathogenic fungus.

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