Show simple item record

dc.contributor.authorUlluwishewa, D.
dc.contributor.authorWang, L.
dc.contributor.authorPereira, C.
dc.contributor.authorFlynn, S.
dc.contributor.authorCain, E.
dc.contributor.authorStick, S.
dc.contributor.authorReen, F.
dc.contributor.authorRamsay, Joshua
dc.contributor.authorO'Gara, Fergal
dc.date.accessioned2017-01-30T15:35:28Z
dc.date.available2017-01-30T15:35:28Z
dc.date.created2016-06-16T19:30:16Z
dc.date.issued2016
dc.identifier.citationUlluwishewa, D. and Wang, L. and Pereira, C. and Flynn, S. and Cain, E. and Stick, S. and Reen, F. et al. 2016. Dissecting the regulation of bile-induced biofilm formation in Staphylococcus aureus. Microbiology. 162: pp. 1398-1406.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/47749
dc.identifier.doi10.1099/mic.0.000317
dc.description.abstract

Aspiration of bile into the cystic fibrosis (CF) lung has emerged as a prognostic factor for reduced microbial lung biodiversity and the establishment of often fatal, chronic pathogen infections. Staphylococcus aureus is one of the earliest pathogens detected in the lungs of children with CF, and once established as a chronic infection, strategies for its eradication become limited. Several lung pathogens are stimulated to produce biofilms in vitro in the presence of bile. In this study, we further investigated the effects of bile on S. aureus biofilm formation. Most clinical S. aureus strains and the laboratory strain RN4220 were stimulated to form biofilms with sub-inhibitory concentrations of bile. Additionally, we observed bile-induced sensitivity to aminoglycosides, which we exploited in a bursa aurealis transposon screen to isolate mutants reduced in aminoglycoside sensitivity and augmented in bile-induced biofilm formation. We identified five mutants that exhibited hypersensitivity to bile with respect to bile-induced biofilm formation, three of which carried transposon insertions within gene clusters involved in wall teichoic acid (WTA) biosynthesis or transport. Strain TM4 carried an insertion between the divergently oriented tagH-tagG genes, encoding the putative WTA membrane translocation apparatus. Ectopic expression of tagG in TM4 restored a wild-type bile-induced biofilm response, suggesting that reduced translocation of WTA in TM4 induced sensitivity to bile and enhanced bile-induced biofilm formation. We propose that WTA may be important for protecting S. aureus against exposure to bile and that bile-induced biofilm formation may be an evolved response to protect cells from bile-induced cell lysis.

dc.publisherSociety for General Microbiology
dc.titleDissecting the regulation of bile-induced biofilm formation in Staphylococcus aureus.
dc.typeJournal Article
dcterms.source.titleMicrobiology
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access via publisher


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record