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dc.contributor.authorKanyenda, L.
dc.contributor.authorVerdile, Giuseppe
dc.contributor.authorMartins, R.
dc.contributor.authorMeloni, B.
dc.contributor.authorChieng, J.
dc.contributor.authorMastaglia, F.
dc.contributor.authorLaws, S.
dc.contributor.authorAnderton, R.
dc.contributor.authorBoulos, S.
dc.date.accessioned2017-03-15T22:03:33Z
dc.date.available2017-03-15T22:03:33Z
dc.date.created2017-02-24T00:09:08Z
dc.date.issued2014
dc.identifier.citationKanyenda, L. and Verdile, G. and Martins, R. and Meloni, B. and Chieng, J. and Mastaglia, F. and Laws, S. et al. 2014. Is Cholesterol and Amyloid-[beta] Stress Induced CD147 Expression a Protective Response? Evidence that Extracellular Cyclophilin A Mediated Neuroprotection is Reliant on CD147. Journal of Alzheimer's Disease. 39: pp. 545-556.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/49252
dc.description.abstract

The CD147 protein is a ubiquitous multifunctional membrane receptor. Expression of CD147, which is regulated by sterol carrier protein, reportedly modulates amyloid- (A), the neurotoxic peptide implicated in neuronal degeneration in Alzheimer’s disease (AD). Given that high fat/cholesterol is linked to amyloid deposition in AD, we investigated if cholesterol and/or A can alter CD147 expression in rat cortical neuronal cultures. Water-soluble cholesterol and A42 dose-dependentlyincreased CD147 protein expression, but reduced FL-APP protein expression. Cholesterol and A42 treatment also increased lactate dehydrogenase release but to varying degrees. Upregulation of CD147 expression was probably mediated by oxidative stress, as H2O2 (3M) also induced CD147 protein expression in neuronal cultures. In light of these findings, we investigated if CD147 induction was cytoprotective, a compensatory response to injury, or alternatively, a cell death signal. To this end, weused recombinant adenovirus to overexpress human CD147 (in SH-SY5Y cells and primary cortical neurons), and pre-treated cultures with or without recombinant cyclophilin A (rCYPA) protein, prior to A42 exposure.We showed that increased CD147 expression protected against A42, only when rCYPA protein was added to neuronal cultures. Together, our findings reveal potentially important relationships between cholesterol loading, CD147 expression, A toxicity, and the putative involvement of CYPA protein in neuroprotection in AD.

dc.publisherIOS Press
dc.relation.urihttp://iospress.metapress.com/content/d3h15g7rh2473202/
dc.subjectAlzheimer’s disease
dc.subjectA42
dc.subjectcyclophilin A
dc.subjectneuroprotection
dc.subjectcholesterol
dc.subjectCD147
dc.titleIs Cholesterol and Amyloid-[beta] Stress Induced CD147 Expression a Protective Response? Evidence that Extracellular Cyclophilin A Mediated Neuroprotection is Reliant on CD147
dc.typeJournal Article
dcterms.source.volume39
dcterms.source.startPage545
dcterms.source.endPage556
dcterms.source.issn1387-2877
dcterms.source.titleJournal of Alzheimer's Disease
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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