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    HLA typing by sequence-specific primers

    Access Status
    Fulltext not available
    Authors
    Bunce, Michael
    Passey, B.
    Date
    2013
    Type
    Book Chapter
    
    Metadata
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    Citation
    Bunce, M. and Passey, B. 2013. HLA typing by sequence-specific primers. In Transplantation Immunology: Methods and Protocols, pp. 147-159. Springer.
    Source Title
    Transplantation Immunology: Methods and Protocols
    DOI
    10.1007/978-1-62703-493-7_8
    ISBN
    9781627034920
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/51990
    Collection
    • Curtin Research Publications
    Abstract

    HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise. © Springer Science+Business Media, LLC 2013.

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