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dc.contributor.authorBunce, Michael
dc.contributor.authorPassey, B.
dc.identifier.citationBunce, M. and Passey, B. 2013. HLA typing by sequence-specific primers. In Transplantation Immunology: Methods and Protocols, pp. 147-159. Springer.

HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise. © Springer Science+Business Media, LLC 2013.

dc.publisherHumana Press
dc.titleHLA typing by sequence-specific primers
dc.typeBook Chapter
dcterms.source.titleTransplantation Immunology: Methods and Protocols
curtin.departmentDepartment of Environment and Agriculture
curtin.accessStatusFulltext not available

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