HLA typing by sequence-specific primers
| dc.contributor.author | Bunce, Michael | |
| dc.contributor.author | Passey, B. | |
| dc.date.accessioned | 2017-04-28T13:57:05Z | |
| dc.date.available | 2017-04-28T13:57:05Z | |
| dc.date.created | 2017-04-28T09:06:05Z | |
| dc.date.issued | 2013 | |
| dc.identifier.citation | Bunce, M. and Passey, B. 2013. HLA typing by sequence-specific primers. In Transplantation Immunology: Methods and Protocols, pp. 147-159. Springer. | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/51990 | |
| dc.identifier.doi | 10.1007/978-1-62703-493-7_8 | |
| dc.description.abstract |
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise. © Springer Science+Business Media, LLC 2013. | |
| dc.publisher | Humana Press | |
| dc.title | HLA typing by sequence-specific primers | |
| dc.type | Book Chapter | |
| dcterms.source.volume | 1034 | |
| dcterms.source.startPage | 147 | |
| dcterms.source.endPage | 159 | |
| dcterms.source.title | Transplantation Immunology: Methods and Protocols | |
| dcterms.source.isbn | 9781627034920 | |
| curtin.department | Department of Environment and Agriculture | |
| curtin.accessStatus | Fulltext not available |
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