Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
MetadataShow full item record
Mining of mineral resources produces substantial volumes of crushed rock based wastes that are characterised by poor physical structure and hydrology, unstable geochemistry and potentially toxic chemical conditions. Recycling of these substrates is desirable and can be achieved by blending waste with native soil to form a 'novel substrate' which may be used in future landscape restoration. However, these post-mining substrate based 'soils' are likely to contain significant abiotic constraints for both plant and microbial growth. Effective use of these novel substrates for ecosystem restoration will depend on the efficacy of stored topsoil as a potential microbial inoculum as well as the subsequent generation of key microbial soil functions originally apparent in local pristine sites. Here, using both marker gene and shotgun metagenome sequencing, we show that topsoil storage and the blending of soil and waste substrates to form planting substrates gives rise to variable bacterial and archaeal phylogenetic composition but a high degree of metabolic conservation at the community metagenome level. Our data indicates that whilst low phylogenetic conservation is apparent across substrate blends we observe high functional redundancy in relation to key soil microbial pathways, allowing the potential for functional recovery of key belowground pathways under targeted management.
Showing items related by title, author, creator and subject.
Kneller, T.; Harris, Richard; Bateman, A.; Muñoz-Rojas, M. (2018)One of the most critical challenges faced in restoration of disturbed arid lands is the limited availability of topsoil. In post-mining restoration, alternative soil substrates such as mine waste could be an adequate ...
Young calcareous soil chronosequences as a model for ecological restoration on alkaline mine tailings.Cross, Adam; Lambers, H. (2017)Tailings are artificial soil-forming substrates that have not been created by the natural processes of soil formation and weathering. The extreme pH environment and corresponding low availability of some macro- and ...
Muñoz-Rojas, Miriam; Erickson, T.; Dixon, Kingsley; Merritt, D. (2016)© 2016 Society for Ecological Restoration. A thorough knowledge of soil functionality is critical to successful restoration of disturbed ecosystems, and its evaluation involves the assessment of soil properties and processes ...