Oligomerization and toxicity of Aß fusion proteins

Caine, J. and Bharadwaj, P. and Sankovich, S. and Ciccotosto, G. and Streltsov, V. and Varghese, J. 2011. Oligomerization and toxicity of Aß fusion proteins. Biochemical and Biophysical Research Communications. 409 (3): pp. 477-482.

Source Title

Biochemical and Biophysical Research Communications

DOI

10.1016/j.bbrc.2011.05.029

ISSN

0006-291X

School

School of Biomedical Sciences

URI

http://hdl.handle.net/20.500.11937/5565

Collection

Curtin Research Publications

Abstract

This study has found that the Maltose binding protein Aß42 fusion protein (MBP-Aß42) forms soluble oligomers while the shorter MBP-Aß16 fusion and control MBP did not. MBP-Aß42, but neither MBP-Aß16 nor control MBP, was toxic in a dose-dependent manner in both yeast and primary cortical neuronal cells. This study demonstrates the potential utility of MBP-Aß42 as a reagent for drug screening assays in yeast and neuronal cell cultures and as a candidate for further Aß42 characterization. © 2011 .