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    Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells

    Access Status
    Fulltext not available
    Authors
    Guo, Z.
    Li, S.
    Wang, C.
    Xu, J.
    Kirk, B.
    Wu, Jian-Ping
    Liu, Z.
    Xue, W.
    Date
    2017
    Type
    Journal Article
    
    Metadata
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    Citation
    Guo, Z. and Li, S. and Wang, C. and Xu, J. and Kirk, B. and Wu, J. and Liu, Z. et al. 2017. Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells. Journal of Bioactive and Compatible Polymers. 32 (1): pp. 17-31.
    Source Title
    Journal of Bioactive and Compatible Polymers
    DOI
    10.1177/0883911516648969
    ISSN
    0883-9115
    School
    Department of Mechanical Engineering
    URI
    http://hdl.handle.net/20.500.11937/55865
    Collection
    • Curtin Research Publications
    Abstract

    © SAGE Publications. Thermosensitive poly(N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensive understanding of the biosafety of poly(N-isopropylacrylamide) and the interaction of poly(N-isopropylacrylamide) with different cell lines, which has little research until now. In this work, we evaluated the biocompatibility of poly(N-isopropylacrylamide) including cell viability, nitric oxide production, and apoptosis of macrophages RAW264.7, human bronchial epithelial cells, A549, and human umbilical vein endothelial cells in the presence of poly(N-isopropylacrylamide). We have also examined the cellular uptake mechanisms of poly(N-isopropylacrylamide) using endocytic inhibitors and insighted into the intracellular co-localization of poly(N-isopropylacrylamide) using confocal laser scanning microscope. The results showed that poly(N-isopropylacrylamide) had good biocompatibility and could be internalized by these cells. It is macropinocytosis that poly(N-isopropylacrylamide) could be internalized in RAW264.7 cells and caveolae-mediated endocytosis in human bronchi al epithelial cells, A549, and human umbilical vein endothelial cells. In addition, we also evidenced that intracellular poly(N-isopropylacrylamide) was co-localized with lysosome. The study provided important information for the development and clinical applications of poly(N-isopropylacrylamide) in the biomedical field.

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