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dc.contributor.authorGuo, Z.
dc.contributor.authorLi, S.
dc.contributor.authorWang, C.
dc.contributor.authorXu, J.
dc.contributor.authorKirk, B.
dc.contributor.authorWu, Jian-Ping
dc.contributor.authorLiu, Z.
dc.contributor.authorXue, W.
dc.identifier.citationGuo, Z. and Li, S. and Wang, C. and Xu, J. and Kirk, B. and Wu, J. and Liu, Z. et al. 2017. Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells. Journal of Bioactive and Compatible Polymers. 32 (1): pp. 17-31.

© SAGE Publications. Thermosensitive poly(N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensive understanding of the biosafety of poly(N-isopropylacrylamide) and the interaction of poly(N-isopropylacrylamide) with different cell lines, which has little research until now. In this work, we evaluated the biocompatibility of poly(N-isopropylacrylamide) including cell viability, nitric oxide production, and apoptosis of macrophages RAW264.7, human bronchial epithelial cells, A549, and human umbilical vein endothelial cells in the presence of poly(N-isopropylacrylamide). We have also examined the cellular uptake mechanisms of poly(N-isopropylacrylamide) using endocytic inhibitors and insighted into the intracellular co-localization of poly(N-isopropylacrylamide) using confocal laser scanning microscope. The results showed that poly(N-isopropylacrylamide) had good biocompatibility and could be internalized by these cells. It is macropinocytosis that poly(N-isopropylacrylamide) could be internalized in RAW264.7 cells and caveolae-mediated endocytosis in human bronchi al epithelial cells, A549, and human umbilical vein endothelial cells. In addition, we also evidenced that intracellular poly(N-isopropylacrylamide) was co-localized with lysosome. The study provided important information for the development and clinical applications of poly(N-isopropylacrylamide) in the biomedical field.

dc.titleBiocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells
dc.typeJournal Article
dcterms.source.titleJournal of Bioactive and Compatible Polymers
curtin.departmentDepartment of Mechanical Engineering
curtin.accessStatusFulltext not available

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