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    Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia

    Access Status
    Open access via publisher
    Authors
    Daniel, D.
    Lee, S.
    Gan, H.
    Dykes, Gary
    Rahman, S.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Daniel, D. and Lee, S. and Gan, H. and Dykes, G. and Rahman, S. 2016. Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia. Journal of Infection and Public Health.
    Source Title
    Journal of Infection and Public Health
    DOI
    10.1016/j.jiph.2017.02.006
    ISSN
    1876-0341
    School
    School of Public Health
    URI
    http://hdl.handle.net/20.500.11937/56242
    Collection
    • Curtin Research Publications
    Abstract

    © 2017 The Authors. Enterococcus faecalis ranks as one of the leading causes of nosocomial infections. A strong epidemiological link has been reported between E. faecalis inhabiting animals and environmental sources. This study investigates the genetic diversity, antibiotic resistance and virulence determinants in E. faecalis from three sources in Malaysia. A total of 250 E. faecalis isolates were obtained consisting of 120 isolates from farm animals, 100 isolates from water sources and 30 isolates from hospitalized patients. Pulse-field gel electrophoresis-typing yielded 63 pulsotypes, with high diversity observed in all sources (D = =0.901). No pulsotype was common to all the three sources. Each patient room had its own unique PFGE pattern which persisted after six months. Minimum inhibitory concentrations of Vancomycin, Gentamicin, Penicillin, Tetracycline, Nitrofurantoin, Levofloxacin, Ciprofloxacin and Fosfomycin were evaluated. Resistance to Tetracycline was most prevalent in isolates from farm animals (62%) and water sources (49%). Water isolates (86%) had a higher prevalence of the asa1 gene, which encodes for aggregation substance, whereas clinical (78%) and farm animal isolates (87%) had a higher prevalence of the esp gene, encoding a surface exposed protein. This study generates knowledge on the genetic diversity of E. faecalis with antibiotic resistance and virulence characteristics from various sources in Malaysia.

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