Investigating Intracellular Localisation and Cytotoxicity Trends for Neutral and Cationic Iridium Tetrazolato Complexes in Live Cells
Embargo Lift Date
MetadataShow full item record
A family of five neutral cyclometalated iridium(III) tetrazolato complexes and their methylated cationic analogues have been synthesised and characterised. The complexes are distinguished by variations of the substituents or degree of p conjugation on either the phenylpyridine or tetrazolato ligands. The photophysical properties of these species have been evaluated in organic and aqueous media, revealing predominantly a solvatochromic emission originating from mixed metal-to-ligand and ligand-to-ligand charge transfer excited states of triplet multiplicity. These emissions are characterised by typically long excited-state lifetimes (~hundreds of ns), and quantum yields around 5-10% in aqueous media. Methylation of the complexes caused a systematic red-shift of the emission profiles. The behaviour and the effects of the different complexes were then examined in cells. The neutral species localised mostly in the endoplasmic reticulum and lipid droplets, whereas the majority of the cationic complexes localised in the mitochondria. The amount of complexes found within cells does not depend on lipophilicity, which potentially suggests diverse uptake mechanisms. Methylated analogues were found to be more cytotoxic compared to the neutral species, a behaviour that might to be linked to a combination of uptake and intracellular localisation.
This is the peer reviewed version of the following article: Caporale, C. and Bader, C. and Sorvina, A. and Magee, K. and Skelton, B. and Gillam, T. and Wright, P. et al. 2017. Investigating Intracellular Localisation and Cytotoxicity Trends for Neutral and Cationic Iridium Tetrazolato Complexes in Live Cells. Chemistry - A European Journal. 23 (62): pp. 15666–15679, which has been published in final form at 10.1002/chem.201701352This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving at http://olabout.wiley.com/WileyCDA/Section/id-828039.html
Showing items related by title, author, creator and subject.
Werrett, M.; Huff, G.; Muzzioli, S.; Fiorini, V.; Zacchini, S.; Skelton, B.; Maggiore, A.; Malicka, J.; Cocchi, M.; Gordon, K.; Stagni, S.; Massi, Massimiliano (2015)The irreversible reaction of methyl triflate with neutral Re(I) tetrazolato complexes of the type fac-[Re(diim)(CO)3(L)], where diim is either 1,10-phenanthroline or 2,2′-bipyridine and L is a para substituted 5-aryltetrazolate, ...
Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes.Bader, C.; Carter, E.; Safitri, A.; Simpson, Peter; Wright, P.; Stagni, S.; Massi, M.; Lay, P.; Brooks, D.; Plush, S. (2016)Fourier transform infrared (FTIR) microspectroscopy and confocal imaging have been used to demonstrate that the neutral rhenium(i) tricarbonyl 1,10-phenanthroline complex bound to 4-cyanophenyltetrazolate as the ancillary ...
Ligand-induced structural, photophysical, and electrochemical variations in tricarbonyl rhenium(I) tetrazolato complexesWright, Phillip; Affleck, M.; Muzzioli, S.; Skelton, B.; Raiteri, Paolo; Silvester, Debbie; Stagni, S.; Massi, Massimiliano (2013)Treatment of [Re(CO)5X] (X = Cl, Br) with 2-(2-tert-butyltetrazol-5-yl)pyridine yielded neutral mononuclear complexes by exchange of two CO ligands for the chelating tetrazolato ligand. Treatment of [Re(CO)5Br] with ...