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dc.contributor.authorChandler, J.
dc.contributor.authorGandhi, Neha
dc.contributor.authorMancera, Ricardo
dc.contributor.authorSmith, G.
dc.contributor.authorElizur, A.
dc.contributor.authorVentura, T.
dc.date.accessioned2017-09-27T10:20:41Z
dc.date.available2017-09-27T10:20:41Z
dc.date.created2017-09-27T09:48:05Z
dc.date.issued2017
dc.identifier.citationChandler, J. and Gandhi, N. and Mancera, R. and Smith, G. and Elizur, A. and Ventura, T. 2017. Understanding insulin endocrinology in decapod crustacea: Molecular modelling characterization of an insulin-binding protein and insulin-like peptides in the eastern spiny lobster, Sagmariasus verreauxi. International Journal of Molecular Science. 18 (9): Article ID 1832.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/56748
dc.identifier.doi10.3390/ijms18091832
dc.description.abstract

The insulin signalling system is one of the most conserved endocrine systems of Animalia from mollusc to man. In decapod Crustacea, such as the Eastern spiny lobster, Sagmariasus verreauxi (Sv) and the red-claw crayfish, Cherax quadricarinatus (Cq), insulin endocrinology governs male sexual differentiation through the action of a male-specific, insulin-like androgenic gland peptide (IAG). To understand the bioactivity of IAG it is necessary to consider its bio-regulators such as the insulin-like growth factor binding protein (IGFBP). This work has employed various molecular modelling approaches to represent S. verreauxi IGFBP and IAG, along with additional Sv-ILP ligands, in order to characterise their binding interactions. Firstly, we present Sv-and Cq-ILP2: neuroendocrine factors that share closest homology with Drosophila ILP8 (Dilp8). We then describe the binding interaction of the N-terminal domain of Sv-IGFBP and each ILP through a synergy of computational analyses. In-depth interaction mapping and computational alanine scanning of IGFBP_N’ highlight the conserved involvement of the hotspot residues Q 67 , G 70 , D 71 , S 72 , G 91 , G 92 , T 93 and D 94 . The significance of the negatively charged residues D 71 and D 94 was then further exemplified by structural electrostatics. The functional importance of the negative surface charge of IGFBP is exemplified in the complementary electropositive charge on the reciprocal binding interface of all three ILP ligands. When examined, this electrostatic complementarity is the inverse of vertebrate homologues, such physicochemical divergences elucidate towards ligand-binding specificity between Phyla.

dc.publisherMDPI AG
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleUnderstanding insulin endocrinology in decapod crustacea: Molecular modelling characterization of an insulin-binding protein and insulin-like peptides in the eastern spiny lobster, Sagmariasus verreauxi
dc.typeJournal Article
dcterms.source.volume18
dcterms.source.number9
dcterms.source.issn1661-6596
dcterms.source.titleInternational Journal of Molecular Science
curtin.note

Published by MDPI Publishing

curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access


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