Development and usage of protein microarrays for the quantitative measurement of Panton-Valentine leukocidin
MetadataShow full item record
Staphylococcus aureus is a human pathogen that can harbour several genes encoding exotoxins including leukocidins. A clinically most relevant factor is Panton-Valentine leukocidin (PVL) because of its association with chronic, recurrent or severe skin and soft tissue infections. In this study an antibody array was designed and used to obtain an overview about the in vitro PVL expression levels of 266 clinical isolates of MRSA as well as of MSSA belonging to a wide variety of clonal complexes. For that purpose, a novel precipitation based method was used. Unknown PVL concentrations were determined by mapping the signal intensities for spotted monoclonal antibodies to calibration curves that resulted from experiments with known concentrations of recombinant LukF-PV. In most cases, isolates belonging to one clonal complex (CC) showed similar PVL expressions. However, there were also CCs with widely varying PVL concentrations. First analyses, based on in vitro PVL measurements, showed low PVL concentrations in isolates from severe and fatal conditions that are not associated with PVL, such as sepsis, while isolates from skin and soft tissue infections yielded higher concentrations. Agr-group I and IV isolates generally produced more PVL than isolates from agr-groups II and III. The few isolates harbouring the gene encoding toxic shock syndrome toxin (tst1) were particularly low level PVL producers. However, these issues warrant further studies. The method described herein allows rapid quantification of expressed proteins such as PVL in collections of clinical isolates in order to correlate with clinical or genotypic data with a potential for further parallelisation
Showing items related by title, author, creator and subject.
Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalisCarville, K.; Bowman, J.; Lehmann, Deborah; Riley, T. (2007)pmc logo image Logo of jcm Note: Performing your original search, comparison nasal swabs lehmann, in PubMed Central will retrieve 7 citations. Journal List > J Clin Microbiol > v.45(1); Jan 2007 Abstract ...
Temporal changes in Plasmodium falciparum anti-malarial drug sensitivity in vitro and resistance-associated genetic mutations in isolates from Papua New GuineaKoleala, T.; Karl, S.; Laman, M.; Moore, Brioni; Benjamin, J.; Barnadas, C.; Robinson, L.; Kattenberg, J.; Javati, S.; Wong, R.; Rosanas-Urgell, A.; Betuela, I.; Siba, P.; Mueller, I.; Davis, T. (2015)Background: In northern Papua New Guinea (PNG), most Plasmodium falciparum isolates proved resistant to chloroquine (CQ) in vitro between 2005 and 2007, and there was near-fixation of pfcrt K76T, pfdhfr C59R/S108N and ...
Antimicrobial resistance in gram-positive cocci isolated from poultry in Western Australia : an assessment of poultry meat as a vehicle for the transmission of resistant strains via the food chain.Bertolatti, Dean (2002)The aim of this study was to examine whether Gram-positive cocci isolated from processed poultry in Western Australia provided a potential risk for the transfer of antimicrobial-resistant organisms to humans via commercially ...