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dc.contributor.authorPetry, E.
dc.contributor.authorCruzat, Vinicius
dc.contributor.authorHeck, T.
dc.contributor.authorLeite, J.
dc.contributor.authorde Bittencourt Jr, P.
dc.contributor.authorTirapegui, J.
dc.date.accessioned2017-01-30T10:49:18Z
dc.date.available2017-01-30T10:49:18Z
dc.date.created2015-01-14T20:00:46Z
dc.date.issued2014
dc.identifier.citationPetry, E. and Cruzat, V. and Heck, T. and Leite, J. and de Bittencourt Jr, P. and Tirapegui, J. 2014. Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways. Life Sciences. 94 (2): pp. 130-136.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/5924
dc.identifier.doi10.1016/j.lfs.2013.11.009
dc.description.abstract

Aims: We hypothesized that oral L-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1). Main methods: Adult male Wistar rats were 8-week trained (60-min/day, 5 days/week) on a treadmill. During the last 21 days, the animals were supplemented with either L-alanyl-L-glutamine dipeptide (1.5 g/kg, DIP) or a solution containing the amino acids L-glutamine (1 g/kg) and L-alanine (0.67 g/kg) in their free form(GLN + ALA) or water (controls). Key findings: Plasma from both DIP- and GLN + ALA-treated animals showed higher L-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, L-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p < 0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN + ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1. Significance: In trained rats, oral supplementation with DIP or GLN + ALA solution increased the expression of muscle HSP70, favored muscle L-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.

dc.publisherElsevier
dc.subjectHSF1
dc.subjectGlutathione
dc.subjectOxidative stress
dc.subjectHSP70
dc.subjectExercise
dc.titleAlanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways
dc.typeJournal Article
dcterms.source.volume94
dcterms.source.number2
dcterms.source.startPage130
dcterms.source.endPage136
dcterms.source.issn0024-3205
dcterms.source.titleLife Sciences
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access via publisher


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