Characterization of narrow-leaf lupin (Lupinus angustifolius L.) recombinant major allergen IgE-binding proteins and the natural ß-conglutin counterparts in sweet lupin seed species
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© 2017 Elsevier Ltd ß-conglutin has been identified as a major allergen for Lupinus angustifolius seeds. The aim of this study was to evaluate the binding of IgE to five recombinant ß-conglutin isoforms (rß) that we overexpressed and purified and to their natural counterparts in different lupin species and cultivars. Western blotting suggested ß-conglutins were the main proteins responsible for the IgE reactivity of the lupin species and cultivars. Newly identified polypeptides from “sweet lupin” may constitute a potential new source of primary or cross-reactive sensitization to lupin, particularly to L. albus and L. angustifolius seed proteins. Several of them exhibited qualitative and quantitative differences in IgE-binding among these species and cultivars, mainly in sera from atopic patients that react to lupin rather than peanut. IgE-binding was more consistent to recombinant ß2 than to any of the other isoforms, making this protein a potential candidate for diagnosis and immunotherapy.
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Characterization of narrow-leaf lupin (Lupinus angustifolius L.) recombinant major allergen IgE-binding proteins and the natural ß-conglutin counterparts in sweet lupin seed speciesJimenez-Lopez, J.; Foley, R.; Brear, E.; Clarke, V.; Lima-Cabello, E.; Florido, J.; Singh, Karam; Alché, J.; Smith, P. (2018)© 2017 Elsevier Ltd ß-conglutin has been identified as a major allergen for Lupinus angustifolius seeds. The aim of this study was to evaluate the binding of IgE to five recombinant ß-conglutin isoforms (rß) that we ...
Narrow-leafed lupin (Lupinus angustifolius L.) ß-conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory-related disease ameliorationLima-Cabello, E.; Alche, V.; Foley, R.; Andrikopoulos, S.; Morahan, G.; Singh, Karam; Alche, J.; Jimenez-Lopez, J. (2017)© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Scope: We have investigated the potential use of ß-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment. Methods and ...
Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approachesFoley, R.; Jimenez-Lopez, J.; Kamphuis, L.; Hane, James; Melser, S.; Singh, K. (2015)Background - The major proteins in lupin seeds are conglutins that have primary roles in supplying carbon, sulphur and nitrogen and energy for the germinating seedling. They fall into four families; α, β, γ and δ. Interest ...