Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome
dc.contributor.author | Chhabra, Sagar | |
dc.contributor.author | Brazil, Dina | |
dc.contributor.author | Morrissey, John | |
dc.contributor.author | Burke, James | |
dc.contributor.author | O'Gara, Fergal | |
dc.contributor.author | Dowling, David | |
dc.date.accessioned | 2017-01-30T10:54:38Z | |
dc.date.available | 2017-01-30T10:54:38Z | |
dc.date.created | 2014-03-30T20:00:53Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Chhabra, Sagar and Brazil, Dina and Morrissey, John and Burke, James and O'Gara, Fergal and Dowling, David. 2013. Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome. Microbiology Open. 2 (5): pp. 717-724. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/6682 | |
dc.identifier.doi | 10.1002/mbo3.110 | |
dc.description.abstract |
Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8–77.1 mmol/L and 27.6–38.16 µg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil. | |
dc.publisher | John Wiley & Sons LTD | |
dc.subject | MPS | |
dc.subject | pyrroloquinoline quinone | |
dc.subject | Glucose dehydrogenase | |
dc.subject | soil metagenome | |
dc.title | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome | |
dc.type | Journal Article | |
dcterms.source.volume | 2 | |
dcterms.source.number | 5 | |
dcterms.source.startPage | 717 | |
dcterms.source.endPage | 724 | |
dcterms.source.issn | 2045-8827 | |
dcterms.source.title | Microbiology Open | |
curtin.department | ||
curtin.accessStatus | Open access via publisher |