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    Isolation and characterization of urease-producing bacteria from tropical peat

    Access Status
    Fulltext not available
    Authors
    Phang, I.
    Chan, Stephanie
    Wong, K.
    Lau, S.
    Date
    2018
    Type
    Journal Article
    
    Metadata
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    Citation
    Phang, I. and Chan, S. and Wong, K. and Lau, S. 2018. Isolation and characterization of urease-producing bacteria from tropical peat. Biocatalysis And Agricultural Biotechnology. 13: pp. 168-175.
    Source Title
    Biocatalysis And Agricultural Biotechnology
    DOI
    10.1016/j.bcab.2017.12.006
    ISSN
    1878-8181
    School
    Curtin Malaysia
    URI
    http://hdl.handle.net/20.500.11937/67201
    Collection
    • Curtin Research Publications
    Abstract

    Urease were known to catalyze the conversion of urea to ammonia and carbon dioxide. Microbial urease has demonstrated its benefits in wide biotechnological, agricultural, medicinal and engineering application. There are number of diverse microbial species contribute to urease activity in different natural habitats like soil, ocean and in various geological formation. For this study, urease bacteria were screened and isolate from acidic peat in Sarawak, Malaysia. Five distinct and diverse bacterial strains that were able to produce urease constitutively were selected to be characterized with respect to morphology, biochemical test, growth conditions and urease activity. The selected strain showed their capability to precipitate calcium carbonate (CaCO 3 ). Hence, the isolates could be potential source of acid ureases that can be use in various industrial utilizations. 16S rRNA sequencing and phylogenetic analysis found that the selected isolates belong to the genus of Bacillus.

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