Lupin seed γ-conglutin: Extraction and purification methods - A review
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Background: Lupin, the largest legume crop in Australia, is gaining global attention because of its unique protein Î³-conglutin, which has shown promise as a nutraceutical for controlling blood glucose level and thus reducing the risk of type II diabetes development. Type II diabetes is a chronic condition affecting millions of individuals worldwide, which urgently requires natural side effect free therapies as alternatives to currently used drugs. Purification of Î³-conglutin opens up a new avenue for high-value products from lupin seeds as nutraceuticals, the market for which is predicted to reach US$ 250 billion by 2018 (Dutta, Mahabir, & Pathak, 2013). Scope and approach: Previously, several research groups have reported trials on extracting and purifying proteins from lupin seed. However, most of these methods have focussed on protein isolates as food ingredients. Very few reports have aimed to purify Î³-conglutin from the total proteins, but the methods reported are time-consuming and unsuitable for commercial scale production of high purity Î³-conglutin due to the involvement of many processing steps for nutraceutical application. Hence there is a need to fully understand all reported Î³-conglutin extraction and purification processes in terms of their advantages and limitations, so that an effective scalable purification process for nutraceutical grade Î³-conglutin may be designed in the future. Key findings and conclusions: This article reviews reported extraction and purification methods for Î³-conglutin, to provide a basis for the development of novel purification technique/process for this potentially highly valuable protein.
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Mane, S.; Bringans, S.; Johnson, Stuart; Pareek, Vishnu; Utikar, Ranjeet (2017)© 2017 Elsevier B.V. A simple, selective and accurate reverse phase HPLC method was developed for detection and quantitation of ?-conglutin from lupin seed extract. A linear gradient of water and acetonitrile containing ...
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Mane, Sharmilee (2017)This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with ...