Isolation and purification of protein (Conglutin-γ) from lupin seeds
Access Status
Open access
Authors
Mane, Sharmilee
Date
2017Supervisor
Dr Ranjeet Utikar
Type
Thesis
Award
PhD
Metadata
Show full item recordFaculty
Science and Engineering
School
Chemical Engineering
Collection
Abstract
This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques.
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