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    Isolation and purification of protein (Conglutin-γ) from lupin seeds

    Mane S 2017.pdf (9.473Mb)
    Access Status
    Open access
    Authors
    Mane, Sharmilee
    Date
    2017
    Supervisor
    Dr Ranjeet Utikar
    Type
    Thesis
    Award
    PhD
    
    Metadata
    Show full item record
    Faculty
    Science and Engineering
    School
    Chemical Engineering
    URI
    http://hdl.handle.net/20.500.11937/68387
    Collection
    • Curtin Theses
    Abstract

    This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques.

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