Isolation and purification of protein (Conglutin-γ) from lupin seeds
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This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques.
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Narrow-leafed lupin (Lupinus angustifolius) ß1- and ß6-conglutin proteins exhibit antifungal activity, protecting plants against necrotrophic pathogen induced damage from sclerotinia sclerotiorum and phytophthora nicotianaeJimenez-Lopez, J.; Melser, S.; DeBoer, K.; Thatcher, L.; Kamphuis, Lars; Foley, R.; Singh, Karam (2016)Vicilins (7S globulins) are seed storage proteins and constitute the main protein family in legume seeds, particularly in narrow-leafed lupin (Lupinus angustifolius L.; NLL), where seven vicilin genes, called ß1- to ...
Mane, Sharmilee; Johnson, Stuart; Duranti, M.; Pareek, Vishnu; Utikar, Ranjeet (2018)Background: Lupin, the largest legume crop in Australia, is gaining global attention because of its unique protein Î³-conglutin, which has shown promise as a nutraceutical for controlling blood glucose level and thus ...
Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approachesFoley, R.; Jimenez-Lopez, J.; Kamphuis, L.; Hane, James; Melser, S.; Singh, K. (2015)Background - The major proteins in lupin seeds are conglutins that have primary roles in supplying carbon, sulphur and nitrogen and energy for the germinating seedling. They fall into four families; α, β, γ and δ. Interest ...