Isolation and purification of protein (Conglutin-γ) from lupin seeds
| dc.contributor.author | Mane, Sharmilee | |
| dc.contributor.supervisor | Dr Ranjeet Utikar | en_US |
| dc.date.accessioned | 2018-06-21T01:06:42Z | |
| dc.date.available | 2018-06-21T01:06:42Z | |
| dc.date.issued | 2017 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/68387 | |
| dc.description.abstract |
This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques. | en_US |
| dc.publisher | Curtin University | en_US |
| dc.title | Isolation and purification of protein (Conglutin-γ) from lupin seeds | en_US |
| dc.type | Thesis | en_US |
| dcterms.educationLevel | PhD | en_US |
| curtin.department | Chemical Engineering | en_US |
| curtin.accessStatus | Open access | en_US |
| curtin.faculty | Science and Engineering | en_US |