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    Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

    Access Status
    Fulltext not available
    Authors
    Buzid, A.
    Luong, J.
    Reen, F.
    O'Gara, Fergal
    Glennon, J.
    McGlacken, G.
    Date
    2018
    Type
    Journal Article
    
    Metadata
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    Citation
    Buzid, A. and Luong, J. and Reen, F. and O'Gara, F. and Glennon, J. and McGlacken, G. 2018. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode. Methods in Molecular Biology. 1673: pp. 107-116.
    Source Title
    Methods in Molecular Biology
    DOI
    10.1007/978-1-4939-7309-5_9
    ISSN
    1940-6029
    School
    School of Pharmacy and Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/69785
    Collection
    • Curtin Research Publications
    Abstract

    As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

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