Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
MetadataShow full item record
As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.
Showing items related by title, author, creator and subject.
Berwick, Lyndon (2009)The analytical capacity of MSSV pyrolysis has been used to extend the structural characterisation of aquatic natural organic matter (NOM). NOM can contribute to various potable water issues and is present in high ...
Zhang, W.; Hao, Hong; Wu, J.; Li, J.; Ma, H.; Li, C. (2017)Ultrasonic Guided Wave (UGW)-based method is a very promising nondestructive testing (NDT) technique for detecting defects in elongated structures, such as pipelines, railway tracks and elongated bars. Minor damage in ...
Rohani-Mehdiabadi, Bijan (1998)In recent years, millions of customers all over the world have been subscribing to mobile telephony services which are based on modern digital transmission. At the high transmission bit rates that these systems use, the ...