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dc.contributor.authorBuzid, A.
dc.contributor.authorLuong, J.
dc.contributor.authorReen, F.
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorGlennon, J.
dc.contributor.authorMcGlacken, G.
dc.date.accessioned2018-08-08T04:42:19Z
dc.date.available2018-08-08T04:42:19Z
dc.date.created2018-08-08T03:50:37Z
dc.date.issued2018
dc.identifier.citationBuzid, A. and Luong, J. and Reen, F. and O'Gara, F. and Glennon, J. and McGlacken, G. 2018. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode. Methods in Molecular Biology. 1673: pp. 107-116.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/69785
dc.identifier.doi10.1007/978-1-4939-7309-5_9
dc.description.abstract

As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane.

dc.publisherHumana Press
dc.titleRapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
dc.typeJournal Article
dcterms.source.volume1673
dcterms.source.startPage107
dcterms.source.endPage116
dcterms.source.issn1940-6029
dcterms.source.titleMethods in Molecular Biology
curtin.departmentSchool of Pharmacy and Biomedical Sciences
curtin.accessStatusFulltext not available


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