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    Luminescent protein staining with Re(i) tetrazolato complexes

    267756.pdf (1.070Mb)
    Access Status
    Open access
    Authors
    Fiorini, V.
    Bergamini, L.
    Monti, N.
    Zacchini, S.
    Plush, S.
    Massi, Massimiliano
    Hochkoeppler, A.
    Stefan, A.
    Stagni, S.
    Date
    2018
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Fiorini, V. and Bergamini, L. and Monti, N. and Zacchini, S. and Plush, S. and Massi, M. and Hochkoeppler, A. et al. 2018. Luminescent protein staining with Re(i) tetrazolato complexes. Dalton Transactions. 47 (28): pp. 9400-9410.
    Source Title
    Dalton Transactions
    DOI
    10.1039/c8dt02052c
    ISSN
    1477-9226
    School
    School of Molecular and Life Sciences (MLS)
    Remarks

    This document is the Accepted Manuscript version of a Published Work that appeared in final form in Dalton Transactions, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see 10.1039/c8dt02052c

    URI
    http://hdl.handle.net/20.500.11937/69930
    Collection
    • Curtin Research Publications
    Abstract

    Within the general framework of our past and current studies dealing with the investigation of the photophysical properties and the biological behavior of the family of tetrazolato and tetrazole Re(i) complexes, we have endeavored to investigate their potential in the luminescent staining of proteins purified by acrylamide gel electrophoresis. With the aim to provide the first examples of luminescent Re(i) complexes to be exploited for this specific purpose, we have designed and prepared four new Re(i)-based species with the general formula fac-[Re(CO)3(N^N)(Tph)]2-/0, where Tph is the 5-(phenyl)tetrazolato anion and N^N is in turn represented by bathophenanthroline disulfonate (BPS), bathocuproine disulfonate (BCS) or by the SO3-free bathocuproine (BC). In this latter case, the neutral complex fac-[Re(CO)3(BC)(Tph)] served as a model species for the characterization of the former disulfonate complexes. Its cationic analogue fac-[Re(CO)3(BC)(Tph-Me)]+was also prepared by a straightforward methylation reaction. All complexes displayed bright phosphorescence in organic media and, relative to their water solubility, the dianionic species fac-[Re(CO)3(BPS)(Tph)]2-and fac-[Re(CO)3(BCS)(Tph)]2-were also highly emissive in aqueous solution. The sulfonate groups played a key role in promoting and significantly enhancing the luminescent staining performances of both the Re(i) complexes fac-[Re(CO)3(BPS)(Tph)]2-and fac-[Re(CO)3(BCS)(Tph)]2-for proteins. Highlighting a response superior to that of Coomassie Blue and comparable to the one obtained by the well-known silver staining method, these dianionic Re(i)-complexes could efficiently detect up to 50 ng of pure Bovine Serum Albumin (BSA), as well as all proteins found in a Standard Protein Marker mix and from a total protein extract. A lower but still good response for luminescent protein staining was surprisingly obtained by employing the-SO3-free neutral and cationic complexes fac-[Re(CO)3(BC)(Tph)] and fac-[Re(CO)3(BC)(Tph-Me)]+, respectively. These preliminary results open up new possibilities for the further widening of the use of Re(i)-based complexes as luminescent protein staining agents.

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