Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging.
MetadataShow full item record
Funding and Sponsorship
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
Showing items related by title, author, creator and subject.
The Maia detector array and x-ray fluorescence imaging system: Locating rare precious metal phases in complex samplesRyan, Chris; Siddons, D.; Kirkham, R.; Li, Z.; De Jonge, M.; Paterson, D.; Cleverley, J.; Kuczewski, A.; Dunn, P.; Jensen, M.; De Geronimo, G.; Howard, D.; Godel, B.; Dyl, K.; Fisher, L.; Hough, R.; Barnes, S.; Bland, Phil; Moorhead, G.; James, S.; Spiers, K.; Falkenberg, G.; Boesenberg, U.; Wellenreuther, G. (2013)X-ray fluorescence images acquired using the Maia large solid-angle detector array and integrated real-time processor on the X-ray Fluorescence Microscopy (XFM) beamline at the Australian Synchrotron capture fine detail ...
Ryan, Chris; Siddons, D.; Kirkham, R.; Li, Z.; De Jonge, M.; Paterson, D.; Kuczewski, A.; Howard, D.; Dunn, P.; Falkenberg, G.; Boesenberg, U.; De Geronimo, G.; Fisher, L.; Halfpenny, A.; Lintern, M.; Lombi, E.; Dyl, K.; Jensen, M.; Moorhead, G.; Cleverley, J.; Hough, R.; Godel, B.; Barnes, S.; James, S.; Spiers, K.; Alfeld, M.; Wellenreuther, G.; Vukmanovic, Z.; Borg, S. (2014)Motivated by the challenge of capturing complex hierarchical chemical detail in natural material from a wide range of applications, the Maia detector array and integrated realtime processor have been developed to acquire ...
Vaughan, J.; Reid, Brodie; Ramchandani, S.; Wright, Phillip; Muzzioli, S.; Skelton, B.; Raiteri, Paolo; Brown, David; Stagni, S.; Massi, Massimiliano (2013)The photophysical and photochemical properties of the new tricarbonyl rhenium(I) complexes bound to N-heterocyclic carbene ligands (NHC), fac-[Re(CO)3(N^C)X] (N^C = 1-phenyl-3-(2-pyridyl)imidazole or 1-quinolinyl-3-(2-p ...