Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria
dc.contributor.author | Kose, Sureyya | |
dc.contributor.author | Grice, Kliti | |
dc.contributor.author | Orsi, W. | |
dc.contributor.author | Ballal, M. | |
dc.contributor.author | Coolen, Marco | |
dc.date.accessioned | 2018-08-08T04:44:11Z | |
dc.date.available | 2018-08-08T04:44:11Z | |
dc.date.created | 2018-08-08T03:50:59Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Kose, S. and Grice, K. and Orsi, W. and Ballal, M. and Coolen, M. 2018. Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria. Scientific Reports. 8 (1): Article ID 11218. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/70253 | |
dc.identifier.doi | 10.1038/s41598-018-29571-8 | |
dc.description.abstract |
There is growing evidence for bacteria playing a role in the pathogenesis and formation of pigmented gallstones from humans. These studies mainly involved cultivation of gallstone-associated bacteria and 16S rRNA profiling, providing an indirect link between processes involved in gallstone formation by the bacteria in-situ. Here, we provide functional metagenomic evidence of a range of genes involved in bile stress response, biofilm formation, and anaerobic energy metabolism by Gram-negative Klebsiella in pigmented gallstones from a 76-year-old male patient. Klebsiella was also present in one cholesterol-type stone in a 30-year-old female patient who had additional cholesterol gallstones characterised by Gram-positive bacteria. Pigmented stones further revealed a predominance of genes involved in carbohydrate metabolism, whilst cholesterol stones indicated a profile dominanted by protein metabolism possibly reflecting known chemical differences between Gram-negative and Gram-positive biofilm matrices. Archaeal genes were not detected. Complementary carbon and hydrogen isotopic analyses of cholesterol within the patients' stones revealed homogeneity, suggesting a common diet or cholesterol biosynthesis pathway that has little influence on microbial composition. This pilot study provides a framework to study microbial processes that play a potential role in gallstone formation across markedly different types of stones and patient backgrounds. | |
dc.publisher | Nature Publishing Group | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria | |
dc.type | Journal Article | |
dcterms.source.volume | 8 | |
dcterms.source.number | 1 | |
dcterms.source.issn | 2045-2322 | |
dcterms.source.title | Scientific Reports | |
curtin.department | School of Earth and Planetary Sciences (EPS) | |
curtin.accessStatus | Open access |