DNA metabarcoding—a new approach to fauna monitoring in mine site restoration
MetadataShow full item record
Funding and Sponsorship
This is the peer reviewed version of the following article: Fernandes, K., van der Heyde, M., Bunce, M., Dixon, K., Harris, R.J., Wardell-Johnson, G. and Nevill, P.G. (2018), DNA metabarcoding—a new approach to fauna monitoring in mine site restoration. Restor Ecol, 26: 1098-1107, which has been published in final form at https://doi.org/10.1111/rec.12868. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Ecological restoration of landscapes is an integral part of the mining process. However, restoration is often constrained by a lack of consistent monitoring approaches. For example, the need for specialist techniques and trapping approaches often limits monitoring of fauna recovery. Application of molecular tools has made important contributions to understanding factors influencing restoration success. Here, we outline advances in next-generation sequencing methods, especially metabarcoding of environmental DNA. These have potential to revolutionize the practical contribution of genetics to the monitoring of fauna in a restoration context. DNA metabarcoding involves the simultaneous characterization of biota using DNA barcodes. It is a powerful method to assess the biodiversity contained within environmental samples (e.g. scats, bulk arthropods, soil, water, and sediment). This review outlines the challenges associated with current approaches to monitoring faunal biodiversity throughout ecological restoration. We also demonstrate how the emergence of DNA metabarcoding could recast monitoring capacity for improved ecological restoration outcomes, while discussing current limitations of a DNA-based approach to biodiversity assessment.
Showing items related by title, author, creator and subject.
Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequencesFernandes, Kristen; Van Der Heyde, Mieke ; Megan, Coghlan; Wardell-Johnson, Grant ; Bunce, Michael ; Harris, Richard ; Nevill, Paul (2019)Invertebrate biomonitoring can reveal crucial information about the status of restoration projects; however, it is routinely underused because of the high level of taxonomic expertise and resources required. Invertebrate ...
Van Der Heyde, Mieke ; Bateman, Bill ; Bunce, Michael ; Wardell-Johnson, Grant ; White, Nicole ; Nevill, Paul (2021)Despite the roles they play in ecosystem function, animals have have long been neglected in the monitoring of ecological restoration. Vertebrate surveys can be time consuming and costly, often requiring multiple methodologies ...
Evaluating restoration trajectories using DNA metabarcoding of ground-dwelling and airborne invertebrates and associated plant communitiesVan Der Heyde, Mieke ; Bunce, Michael ; Dixon, Kingsley ; Fernandes, Kristen; Majer, Jonathan ; Wardell-Johnson, Grant ; White, Nicole ; Nevill, Paul (2022)Invertebrates are important for restoration processes as they are key drivers of many landscape-scale ecosystem functions; including pollination, nutrient cycling and soil formation. However, invertebrates are often ...