DNA metabarcoding—a new approach to fauna monitoring in mine site restoration
|van der Heyde, M.
|Fernandes, K. and van der Heyde, M. and Bunce, M. and Dixon, K. and Harris, R. and Wardell-Johnson, G. and Nevill, P. 2018. DNA metabarcoding—a new approach to fauna monitoring in mine site restoration. Restoration Ecology. 26 (6): pp. 1098-1107.
Ecological restoration of landscapes is an integral part of the mining process. However, restoration is often constrained by a lack of consistent monitoring approaches. For example, the need for specialist techniques and trapping approaches often limits monitoring of fauna recovery. Application of molecular tools has made important contributions to understanding factors influencing restoration success. Here, we outline advances in next-generation sequencing methods, especially metabarcoding of environmental DNA. These have potential to revolutionize the practical contribution of genetics to the monitoring of fauna in a restoration context. DNA metabarcoding involves the simultaneous characterization of biota using DNA barcodes. It is a powerful method to assess the biodiversity contained within environmental samples (e.g. scats, bulk arthropods, soil, water, and sediment). This review outlines the challenges associated with current approaches to monitoring faunal biodiversity throughout ecological restoration. We also demonstrate how the emergence of DNA metabarcoding could recast monitoring capacity for improved ecological restoration outcomes, while discussing current limitations of a DNA-based approach to biodiversity assessment.
|Blackwell Science Inc.
|DNA metabarcoding—a new approach to fauna monitoring in mine site restoration
This is the peer reviewed version of the following article: Fernandes, K., van der Heyde, M., Bunce, M., Dixon, K., Harris, R.J., Wardell-Johnson, G. and Nevill, P.G. (2018), DNA metabarcoding—a new approach to fauna monitoring in mine site restoration. Restor Ecol, 26: 1098-1107, which has been published in final form at https://doi.org/10.1111/rec.12868. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
|School of Molecular and Life Sciences (MLS)