Characterization of polyphenols in Australian sweet lupin (Lupinus angustifolius) seed coat by HPLC-DAD-ESI-MS/MS
MetadataShow full item record
Seeds of the legume lupin (Lupinus spp.) are becoming increasingly important as human food. The seed coat, at ~25% of the whole seed of Lupinus angustifolius (Australian sweet lupin, ASL), is the main by-product of lupin kernel flour production. The primary market for lupin seed coat is low value feed with very limited use in foods. In this study, seed coats of six ASL commercial varieties from two growing sites were sampled for identification and quantification of polyphenols using a high-performance liquid chromatography (HPLC) with diode array detector (DAD) and coupled with a triple quadrupole mass spectrometer which equipped with electrospray ionization source (ESI-MS/MS). Three flavones (apigenin-7-O-ß-apiofuranosyl-6,8-di-C-ß-glucopyranoside, vicenin 2, and apigenin-7-O-ß-glucopyranoside), one isoflavone (genistein) and one dihydroflavonol derivative (aromadendrin-6-C-ß-D-glucopyranosyl-7-O-[ß-D-apiofuranosyl-(1 ? 2)]-O-ß-D-glucopyranoside), and several hydroxybenzoic and hydroxycinnamic acid derivatives were identified. Considerable variations in levels of individual polyphenols were found but apigenin-7-O-ß-apiofuranosyl-6,8-di-C-ß-glucopyranoside was the predominant polyphenol in all samples accounting for 73.08–82.89% of the total free polyphenols. These results suggest that ASL seed coat could be valuable dietary source of polyphenols.
Showing items related by title, author, creator and subject.
Zhong, L.; Fang, Zhongxiang; Wahlqvist, M.; Hodgson, J.; Johnson, Stuart (2019)Extrusion cooking is widely used to improve the functional properties of dietary fibre of food processing by-products. This study investigated the capacity of extrusion cooking to increase the proportion of dietary fibre ...
Zhong, L.; Fang, Zhongxiang; Wahlqvist, M.; Wu, G.; Hodgson, J.; Johnson, Stuart (2018)Background: In recognition of their multiple benefits on environment, food security, and human health, pulses are attracting worldwide attention. The seed coat is a major by-product of pulse processing, and its only markets ...
Khan, M.; Karnpanit, W.; Nasar-Abbas, S.; Huma, Z.; Jayasena, Vijay (2015)Lupin is a nonstarchy grain legume with high protein, dietary fibre and low fat contents. The industrial shift of lupin seed utilisation from feed to food has recently increased the scientific interest to explore its ...