Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
|dc.identifier.citation||Wang, H. and Zhang, R. and Bridle, K. and Jayachandran, A. and Thomas, J. and Zhang, W. and Yuan, J. et al. 2017. Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury. Scientific Reports. 7: Article number 45374.|
Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response.
|dc.publisher||Nature Publishing Group|
|dc.title||Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury|
|curtin.department||WASM: Minerals, Energy and Chemical Engineering (WASM-MECE)|